2025, Vol. 5, Issue 2, Part A
Traditional detection and molecular characterization of salmonella pullorum in broiler chicken in Babylon city- Iraq
Author(s): Mohammed J Hassan and Firas H Al-Bawi
Abstract: Pullorum disease (PD) is one of the most frequent diseases in the world, and it can have terrible effects. There have been money losses in the chicken business. Salmonella enteric subspecies of serovar Gallinarum biovar pullorum causes it. To be sure, you need to culture the bacteria, do biochemical study, and serotype them. The goal of this study was to confirm the presence of bacteria through culture, biochemical and VITEK2 system characterisation, and 16S rRNA gene detection by PCR assay. A total of 100 samples were collected from grill chicken flocks of different ages in 5 districts, including 51 cloacal swabs, 29 visceral organs, and 20 droppings. Sixty-three percent of the samples were found to contain Salmonella colonies, with a higher frequency in visceral organs compared to droppings and cloacal swabs. The samples were identified using selective culture broth and agar with biochemical descriptions. Phylogenetic studies have confirmed the existence of Salmonella pullorum in grill chickens raised in Babylon City, Iraq, according to the latest findings in molecular and genetic research. Twenty isolates, or 100%, of this strain were confirmed using the conventional PCR 16SrRNA gene at 1500 bp. Salmonella pullorum was found in grill flocks in this investigation, which raises concerns about the potential health consequences to other birds that are free-range and not affected.
DOI: 10.22271/letters.2025.v5.i2a.136
Pages: 52-57 | Views: 507 | Downloads: 249
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How to cite this article:
Mohammed J Hassan, Firas H Al-Bawi. Traditional detection and molecular characterization of salmonella pullorum in broiler chicken in Babylon city- Iraq. Zool Entomol Lett 2025;5(2):52-57. DOI: 10.22271/letters.2025.v5.i2a.136
