2025, Vol. 5, Issue 2, Part A
Measurement of the ELISA antibody titers and histopathological changes in the lymphoid tissues of broiler chickens vaccinated with different vaccination programs
Author(s): Mohammed Salih Obaid Al-Mansouri and Hayder Abd AL-Emier Almremdhy
Abstract: One of the approved methods for controlling viral diseases endemic in Iraq, which cause huge economic losses, is the use of vaccines to protect birds against these diseases. This study aimed to evaluate the immune response to two vaccination programs commonly used in Iraq. To achieve this goal, one-day-old Rose 308 broiler chicks were brought in and divided into three groups. The first group was vaccinated in the hatchery at one day old with an intensive vaccination program against avian influenza, Newcastle disease, infectious bronchitis, and infectious bursitis. The chicks in the second group were vaccinated with the same vaccination program in the first group, but on the fifth day of the chicks’ life, with the addition of two doses of Newcastle disease vaccine at the age of 15 and 25. As for the chicks in the third group, they were left without vaccination as a control group. The humoral immunity of the chicks was measured at the age of 10, 20, and 30 days by ELISA, and and the histopathological changes of lymphoid organs was also measured. The results showed no significant differences between the two vaccinated groups in terms of humoral immunity and histopathological changes of lymphoid organs, and there was a clear significant difference with the control group. we conclude that both vaccination programs induce a good humoral immune response. the vaccination programs (time of vaccination, type of vaccine, routs of administration) applied in poultry houses lead to histopathogical changes in lymphoid organ may be used in estimated immune response in chickens. Further future studies need on vaccination programs designs until reach to suitable program can protecting chickens against infectious diseases.
DOI: 10.22271/letters.2025.v5.i2a.134
Pages: 33-41 | Views: 711 | Downloads: 416
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How to cite this article:
Mohammed Salih Obaid Al-Mansouri, Hayder Abd AL-Emier Almremdhy. Measurement of the ELISA antibody titers and histopathological changes in the lymphoid tissues of broiler chickens vaccinated with different vaccination programs. Zool Entomol Lett 2025;5(2):33-41. DOI: 10.22271/letters.2025.v5.i2a.134
