2025, Vol. 5, Issue 1, Part A
Phylogenetic analysis of Salmonella enterica isolates from human and chicken sources in Al-diwaniyah, Iraq
Author(s): Ali Basim Mohammed and Orooba Meteab Faja
Abstract: Background: Salmonella enterica is a global foodborne pathogen causing significant public health concerns. Multidrug-resistant strains pose a serious threat, necessitating effective control measures.Purpose: This study aimed to investigate the genetic diversity and phylogenetic relationships of Salmonella enterica isolates from human and chicken sources in Al-Diwaniyah, Iraq.Study Location and Time: The study was conducted in Al-Diwaniyah, Iraq. The timeframe for the study was not explicitly stated in the text.Sample Collection: Samples were collected from both human stool samples of patients with gastroenteritis and chicken samples (meat and eggs) from local farms and markets.Methods: The study employed conventional PCR amplification of the 16S rRNA gene for molecular identification. PCR products were sequenced and compared with reference sequences in GenBank using BLAST software. Phylogenetic analysis was performed to determine evolutionary relationships.Results: PCR amplification successfully identified Salmonella enterica isolates. Sequencing results revealed genetic variation among the isolates, with similarity ranging from 92.19% to 97.71%. Phylogenetic analysis grouped the isolates into four distinct clusters, indicating genetic diversity within the population.Conclusion: The study highlights the genetic diversity of Salmonella enterica isolates in Al-Diwaniyah, Iraq, emphasizing the need for ongoing surveillance and effective control measures to mitigate public health risks.
DOI: 10.22271/letters.2025.v5.i1a.112
Pages: 16-21 | Views: 79 | Downloads: 43
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How to cite this article:
Ali Basim Mohammed, Orooba Meteab Faja. Phylogenetic analysis of Salmonella enterica isolates from human and chicken sources in Al-diwaniyah, Iraq. Zool Entomol Lett 2025;5(1):16-21. DOI: 10.22271/letters.2025.v5.i1a.112
