2024, Vol. 4, Issue 2, Part A
Molecular detection of Haemonchus spp parasite in sheep in Babylon province
Author(s): Ghaidaa J Kazim and Nawras A Madlol AL-Kaabi
Abstract: The study involved 100 feces samples of sheep, in Babylon Governorate in Iraq. Used for diagnosis of Haemonchus spp, Traditional methods and polymerase chain reaction (PCR), using specific primers and the method was the conventional PCR. Sheep was classified into subgroups according to age, area and sex in this study association of these risk factors with rate of infection. The diagnosis of Haemonchus spp in sheep was determined by method Microscopic examination (Traditional methodes). In the present study, the result of the study revealed that infection percent in sheep was (41%) The result of the study revealed that infection percent in sheep by (Traditional methodes) rate according to the geographical areas infections was highest in Western hamza and Al-Musayyab district which were (50%) and the lower prevalence of Haemonchus spp was in Al-Qassim district which was (30%), The statistical analysis reveals that there was no significant (p>0.05).The infection rate of Haemonchus spp infections by (Traditional methodes) rate was highest in the the (< 1 year) age group which was (44.82%) while the lower prevalence of infection was in the (> 2 years) age group which was (38.70%) no significant (p>0.05) The higher infection rate of Haemonchus spp infection by (Traditional methodes) was at females (42.85%) which was and lower prevalence of infection at males which was (36.66%), no significant effect at (p>0.05). In the present study, the result of the study revealed that infection percent in sheep by (PCR) was (68%), 68 feses samples were positive for Haemonchus worm visible bands in the PCR product at 454 bp. The result of the study revealed that infection percent in sheep by (PCR) rate according to the geographical areas infections was highest in Al-Qassim and Al-Mahaweel districts which were 80% and the lower prevalence of Haemonchus spp was in Al-Musayyab district which was (46.15%), The statistical analysis recorded that there was Significant difference (0.047). The infection rate of Haemonchus spp infections by (PCR) rate was highest in the (> 2 years) age group which was (74.19%), while the lower prevalence of infection was in the (< 1 years) age group which was (62.06%) no significant at (p>0.05). The higher infection rate of Haemonchus spp infection by (PCR) was at males which was (76.66%) and lower prevalence of infection at females which was (64.28%), no significant at (p>0.05).
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How to cite this article:
Ghaidaa J Kazim, Nawras A Madlol AL-Kaabi. Molecular detection of Haemonchus spp parasite in sheep in Babylon province. Zool Entomol Lett 2024;4(2):05-12.
