2024, Vol. 4, Issue 1, Part B
Investigating the presence of E. coli bacteria in broiler chickens and detecting virulence genes (rfbE, eaeA, fliCH7)
Author(s): Dheyaa Ali Aljuhaishi and Firas Hussain Kadim Albawi
Abstract: The study was conducted for the purpose of detecting the genes (rfbE, eaeA, fliCH7) and confirming their diagnosis using polymerase chain reaction testing and biochemical tests. The experiment was carried out in the laboratories of the Pathology and Poultry Diseases Branch at the College of Veterinary Medicine, Al Qasim Green University, during the period from 8/10/2023 to 9/10/2023. (150) samples were collected from chickens suffering from diarrhea and showing clinical symptoms of the infection. They were weighed at one day old (average weight of the chick: 43±2 g) and then transported to the poultry hall. Chickens in houses divided by wooden barriers. Escherichia coli. were first diagnosed by bacteriological methods. Among (150) samples for cases of diarrhea in chickens, (90%) was an isolate of Escherichia coli. bacteria. Then, PCR technology was used to detect the virulence genes (rfbE, eaeA, fliCH7) and to confirm the diagnosis, and the result showed (rfbE, eaeA, fliCH7). In this study, this gene was discovered in (135) isolates from (150) samples of observed chickens carrying the rfb E, eae A, flich7 gene, which represents (90%).
Pages: 120-124 | Views: 501 | Downloads: 254
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How to cite this article:
Dheyaa Ali Aljuhaishi, Firas Hussain Kadim Albawi. Investigating the presence of E. coli bacteria in broiler chickens and detecting virulence genes (rfbE, eaeA, fliCH7) . Zool Entomol Lett 2024;4(1):120-124.
