2022, Vol. 2, Issue 2, Part A
DNA barcoding for deciphering the evolutionary lineages of five insect pests of horticultural crops in Ikorodu local government area of Lagos state, Nigeria
Author(s): Olusola B Sokefun and Gabriel AS Benson
Abstract: Arthropod pest management is dependent on the proper identification of species as quickly as possible. Before now, morphology has been the key methodology, resting basically on the use of established taxonomic keys that have suddenly become plastic because of the introduction of new species and the evolution within old one. The development of the DNA barcoding protocol has proven to be of tremendous help as it relies on sequencing a segment of the cytochrome oxidase 1 gene. Where reference gene sequences exists, this technology is exact and can unambiguously identify pests without errors. The method has become very important with Lepidopteran pests which typically wreak havoc at the larva stages when morphological identification is almost impossible. In this research, we have added first genetic information for Alpenus maculosus, Chilo species, Mussidia species and Sesamia calamistis and have further enriched the available reference database upon which further researches can be built. The study also adds quality information to the molecular ecology of Lepidopteran pests around Ikorodu in Lagos State, Nigeria.
Pages: 31-35 | Views: 314 | Downloads: 149
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How to cite this article:
Olusola B Sokefun, Gabriel AS Benson. DNA barcoding for deciphering the evolutionary lineages of five insect pests of horticultural crops in Ikorodu local government area of Lagos state, Nigeria. Zool Entomol Lett 2022;2(2):31-35.